Basic Informations
C.V
![Description: 1]()
Curriculum Vitae
|
Name:
|
Ahmed Farag Azmy
|
|
|
Position:
|
Asistant lecturer , Microbiology and ImmunologyDepartment, Faculty of Pharmacy, Beni-Suef University.
|
|
Military service:
|
Completed (Jan 2004 – Mar 2005).
|
|
Marital status:
|
Married.
|
|
Nationality:
|
Egyptian.
|
|
Date of Birth:
|
September16, 1981.
|
|
Address:
|
1) Hassan Ibrahim street-Aljazeera, Beni Suef, Egypt.
|
|
Mobile:
|
002 0100 4457502.
|
|
E-mail:
|
ahmed.abdelaziz@pharm.bsu.edu.eg
Drahmedazmy81@gmail.com
|
|
Degrees:
|
Asisstant lecturer
|
|
1) B. Sc. Of Pharmaceutical sciences (Excellent with honor degree), Cairo University, Beni Sueif Branch, 2003.
2) Master degree, 2010, Beni-Suef University.
|
|
Experiences:
|
|
1) Demonstrator of microbiology at the Faculty of Pharmacy, Beni Sueif (Teaching practical course for 2nd, 3rd, and 5th year pharmacy students including introduction to practical pharmaceutical microbiology, practical bacteriology, serology and parasitology), 2005-2011.
2) Participation in the foundation of the nascent department; including routine activities related to purchasing chemicals, glassware, and equipment for the department.
|
|
Fields of research and routine work:
1) Isolation and identification of clinical microbial microorganisms (which include Candida albicans, C. tropicalis, E. coli, Klebsiella spp. Enterobacter spp. Staphylococcus spp.,Methicillin Resistant Staphylococcus Aureus (MRSA) , Pseudomonas spp.) using variety of media and biochemical tests.
2) Performing of antimicrobial susceptibility techniques including disk diffusion method and MIC determination for bacterial clinical isolates.
3) Separation of bacterial plasmid using gel electrophoresis.
|
|
Activities :
|
|
1) Attendance a training course in Tissue culture, Center of science and technology, Alexandria University.
2) Attendance a training course in Bioinformatics, Center of Biotechnology, Faculty of pharmacy, Cairo University.
3) Attendance a training course in Molecular biology and gene Cloning, Center of Biotechnology, Faculty of pharmacy, Cairo University.
|
|
Languages:
1) English: TOEFL cbt score is as follow:
|
Test Date
|
Reading
|
Listening
|
Writing and structure
|
Total
|
|
May,18, 2006
|
19
|
17
|
18
|
180
|
|
|
Computer skills:
1) ICDL equivalent certificate, Supreme Council of Universities, Egypt, August 8, 2006.
2) Advanced Word, Advanced Excel, Advanced PowerPoint equivalent certificate, Supreme Council of Universities, Egypt, June, 2011.
|
Publication:
1- Characterization of Methicillin-Resistant Staphylococcus aureus Strains Isolated from Food and Clinical Samples in Beni-Suef City,Egypt.
Shaaban, A.,Hashem; Magdy, A.,Amin*;Aml, E.,Saafan** and Ahmed, Farag**.
New Egyptian Journal of Microbiology, January 2011; 28:59-74.
2 - The developmental and physiological interactions between free radicals and antioxidant defense system: effect of environmental pollutants
RG Ahmed, S Incerpi, F Ahmed, A Gaber
Journal of Natural Sciences Research 3 (13), 74-110
3- Biodegradation of Malathion by Acinetobacter baumannii Strain AFA Isolated from Domestic Sewage in Egypt
Ahmed F. Azmy , Amal E. Saafan, Tamer M. Essam, Magdy A. Amin, Shaban H. Ahmed
International Journal of Biological, Food, Veterinary and Agricultural Engineering Vol:9(1), 2015, 55-65
Field of specialization:
- Antibiotic resistance.
- Biodegradation.
- Biotechnology.
- Environmental Microbiology.
Master Title
Characterization of Different Types of Methicillin-Resistant Staphylococcus aureus (MRSA) isolated from food and clinical samples
Master Abstract
The study aimed to detect different possible transmission methods of CA-MRSA among our community, such as food as a possible way for transmission or outpatient people admitted to the hospital or in the private clinic.
Based on isolation of Staphylococcus aureus from 210 food samples (milk and milk product, and fast foods from different restaurant), there are 61 Staphylococcus aureus, 50 other species and 99 free samples.
While the 324 clinical samples is taken from different parts of the body for outpatient resulting in 161 Staphylococcus aureus, 76 other species and 87 free specimen.
All isolates are characterized as Staphylococcus aureus by the usual biochemical methods, the isolate that achieve 90% of S.aureus biochemical tests is considered as Staphylococcus aureus. Atypical Staphylococcus aureus (5 coagulase negative S.aureus, 3 DNase negative S.aureus) are isolated from clinical samples.
Methicillin resistance is determined in all Staphylococcus aureus strains by phenotypic and genotypic method. Two phenotypic methods is used oxacillin disk diffusion method and growth on ORSAB medium.
The disk diffusion method was the least sensitive method as they detect 18 MRSA strains and actually they are not resistant. While ORSAB are the most sensitive method achieving high sensitivity 92.8%.
The result of Phenotypic methods is compared with the genotypic method which is considered as gold standard method (detection of mecA gene), detecting only 91 strains carrying the mecA gene.
Antibiogram of the MRSA strains show non multi-resistant pattern confirming that they are community acquired strain, and all of them are sensitive to vancomycin and amikacin.
Due to the appearance of vancomycin resistance among some MRSA isolates in different country, the vancomycin resistance was determined to our isolates by two different methods: disk diffusion method and MIC agar diffusion method. All isolates are vancomycin sensitive and no evidence for resistance or intermediate sensitivity suggesting that it is still the drug of choice in their infections.
From this study, we conclude that:
? Food is a possible pathway for transmission of MRSA either occurred due to contamination with food handlers or from infected animal through ingestion of raw foods.
? Screening of food handlers periodically as they represent a good source of infection, especially for those dangerous one.
? CA-MRSA have high ability to spread in the community so it is advisable to decrease the risk factors increasing their spreading (good hygiene condition and prevent crowd ness).
? Due to the inability to perform genotypic detection of mecA gene by PCR method, it is preferred to use two different phenotypic methods for detection as they have a high sensitivity when used together.
? The antibiotic resistance pattern indicate the possible presence of multi-resistant CA-MRSA. Hence, when MRSA is detected you should take a combination of two different antibiotics.
? Vancomycin is still the most reliable therapeutic agent in our CA-MRSA in our community.
PHD Title
A study on Biodegradation of Some Organophosphorus Xenobiotic Found in Our Environment
PHD Abstract
Bacterial strains capable of degradation of different organophosphorus compounds (OPCs) were isolated from the soil and different aquatic ecosystems (agriculture wastewater, domestic sewage water, and agricultural soil) by an enrichment culture technique. From overall 36 environmental samples 3 bacterial strains show high ability for degradation of various OPCs. They were screened and identified as Acinetobacter baumannii (AFA), Pseudomonas aeruginosa (PA),and Pseudomonas mendocina (PM) based on morphological, biochemical identification and 16S rRNA sequence analysis. Bacterial strains were able to grow in mineral salt medium (MSM) supplemented with malathion (MAL) at a concentration of (100 mg L-1) as a sole carbon and energy source. The degradation profile reveals that Ps.aeruginosa strain PA were able to completely remove MAL within day of inoculation, while A.baumannii strain AFA degraded 84.4% of MAL within 14 day. On the other hand, Ps.mendocina strain PM was the least efficient degrading bacterium, only removed 77.6% of MAL within 14 days. All strains could also degrade other organophosphorus compounds including diazinon, chlorpyrifos and fenitrothion to different degree. The effect of different culture conditions on the degradation of malathion like inoculum density, other carbon or nitrogen sources, temperature and shaking were examined. Degradation of malathion and bacterial cell growth were accelerated when culture media were supplemented with yeast extract, glucose and citrate. The optimum conditions for malathion degradation by the 3 strain were; an inoculum density of 1.5x 1012 cfu ml-1 at 30°C with shaking speed of 150 rpm min-1. From HPLC/MS analysis, malathion monocarboxylic acid (MMC) and malathion dicarboxylic acid (MDC) were the main biodegradation metabolites, so carboxylesterase enzyme may be the responsible enzyme for biodegradation process. A specific polymerase chain reaction primers were designed manually using multiple sequence alignment of the corresponding carboxylesterase enzymes of the 3 species. Sequencing result of amplified PCR product and phylogenetic analysis showed low degree of homology with the other carboxylesterase enzymes of different Pseudomonas and Acinetobacter strains, so we suggested that this enzyme may be a novel esterase enzyme. Isolated bacterial strains may have potential role for use in bioremediation of malathion and OPCs contaminated soil.