Research Article

Phenotypic and genotypic characterization of methicillin resistant Staphylococcus aureus isolated from different sources

Fawzy R. El Seedya, Hala S. H. Salama, Samy A. A., b Eman A. khairyb, Shimaa T. Omarab, Aya A. koraneyb
aDepartment of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt bDepartment of Microbiology and Immunology, National Research Center (NRC), Cairo, Egypt.

Abstract

Food contaminated with multiple antibiotic-resistant S. aureus can be a major threat to the public health.  The purpose of this study was to isolate S. aureus from different food sources, determine their antimicrobial susceptibility as well as detection of mecA gene among some resistant isolates. Out of 125 from food of animal origin samples, 19 S. aureus isolates were recovered, and the antimicrobial susceptibility testing showed a high resistance against kanamycin, penicillin G, oxacillin, erythromycin and tetracycline. All the tested isolates were multiple drug resistant (MDR). Eight out of 19 (15.2%) isolates were phenotypically resistant to oxacillin as well as they were carriers for mecA gene.

Keywords

Staphylococcus. aureus, drug resistance, PCR