TRYPSIN INHIBITORY ACTIVITY AND FRACTIONATION OF PHENOLIC COMPOUNDS AND DEVELOPING PODS AND SEEDS OF COMMON BEAN (PHASEOLUS VULGARIS L.)

Research Abstract

The cultivation of common bean Bronco variety was performed in the Agricultural Research Station - Mallawy to conduct this experiment in order to know changes in the percentage of moisture, trypsin inhibitory activity and the quantity of total phenolic compounds and relation of the seeds development by collection the pods after 10, 20, 30, 40, 50 and 60 days after the anthesis. Our results showed that the percentage of moisture be in the maximum value in the seeds and pods after 10 days of the anthesis as well as the trypsin inhibitory activity low begins in pods and seeds after 10 days of the anthesis and then increase up to a maximum value in the maturity stage and the quantity of total phenolic compounds begins to low in 10 days after the anthesis and then begin to increase until it reaches a maximum value in the maturity stage.

Research Keywords

TIs, HPLC, fractionation of phenolic compounds, TPCs, common bean, salicylic acid (SA), secondary metabolites.