Grape seed oil plus DMBA has a hepatorenal protective effect against damage induced by DMBA in rats.

Research Abstract

7,12-dimethylbenz(a) anthracene (DMBA)has an oxidative damaging effects and grape seed oil (GSO), among its protective mechanisms is the antioxidant properties. Objective: to investigate the hepatorenal protective effects of dietary GSO against liver and renal cytotoxicity induced by DMBA in the rats. Materials and methods: The experimental animals were divided into four groups, the first group was untreated and used as control, the second was treated for 2 weeks with DMBA alone, the third was treated with DMBA plus GSO for 2 weeks, and the fourth group was treated with GSO after DMBA for 2 weeks. Serum liver enzymes, lactate dehydrogenase (LDH), glutamic pyruvic acid transaminase (GPT), glutamic oxaloacetic acid transaminase (GOT), and total plasma proteins (TP S), albumin and globulins were chosen to assess liver function. Urea and creatinine are measured as some of renal function tests. Results: In comparison with control group, DMBA alone significantly increased the serum level of liver GOT,GPT and LDH enzymes and urea and creatinine levels, while total proteins, albumin and globulin was decreased. GSO plus DMBA had decreased significantly, GOT, GPT and LDH and urea while, creatinine was insignificantly changed, also, it had increased total proteins, albumin and globulin when compared with rats subjected to DMBA alone. Administration of GSO whether plus or after DMBA had increased albumin, but, it is significantly lower than in control rats. Conclusion: Treatment with GSO plus DMBA significantly decreases the changes in some hepatic and renal functions induced by DMBA in rats.

Research Keywords

7,12-dimethylbenz(a)anthracene, grape seed oil, liver enzymes, renal function