Basic Informations

C.V

1-Ph.D. degree in Biochemistry Institution: Faculty of Science at Beni-Suef, Cairo University.
Date: 2005
2-Master degree in Biochemistry (M.Sc.) Institution Faculty of Science at Beni-Suef, Cairo University.
Date 1999
13-Bachelor in Biochemistry (B.Sc) from Biochemistry dep. Institution Faculty of Science, Alexandria University. Final graduation Date Very Good with honor 1992
PROFESSIONAL  DETAILS
1- Name of employer:Faculty of Medciene, Zawia University Position: Lecturer
3
From 9/2006till 2014
2-Name of employer: Faculty of Science, Bani-Suef University , Egypt. (Bani-Suef branch becomeindependent university since 1/8/2005)
Position 1- Lecturer in Division of Biochemistry, Department of chemistry, from 2005. 2- Assistant lecturer in Division of Biochemistry, Department of Chemistry from 1999 to 2005 3- Demonstrator (Lecturer assistant) in Division of Biochemistry, Department of Chemistry from 1993 to 1999.

Master Title

Garlic, Vitamin (E), Vitamin (C) and Carbon Tetrachloride Interactions in Oxidative Damage in Rats

Master Abstract

Aim: This study aimed to evaluate hepatoprotective and antioxidative effects of vitamin E,vitamin C and garlic against toxicity exerted by CCl4. These effects were evaluated by determining the levels and activities of phase I and phase II drug-metabolizing enzymes as well as of antioxidants in liver of rats. Methods: The thesis was divided into two experiments, acute and chronic. In acute experiment, all rats were taken single dose of the tested compounds. On the other hand, in the chronic experiment, all animals were taken repeated doses of garlic vitamin C and vitamin E for 13 consecutive days and single dose of CCl4 on the 13th day. In each experiment, the rats were subdivided into 8 groups. Measurments: 1- Hepatic content of microsomal cytochrome P-450 2- Activity of microsomal Aryl hydrocarbon hydroxylase 3- Hepatic content of microsomal cytochrome b-5 4- Activity of microsomal NADPH cytochrome-C reductase 5- Activity of microsomal DMN-N demethylase I 6- Hepatic content of cytosolic GSH 7- Activity of cytosolic GST 8- Microsomal content of TBARs 9- Activity of plasma ALT 10- Activity of plasma AST 11- Hepatic content of microsomal proteins. Results: Repeated doses of vitamin C, vitamin E and garlic attenuated the inhibitory effect of CCl4 on microsomal cytochrome P-450. Single and repeated doses of vitamin E had the most positive effects on GSH content of liver. On the other side, repeated doses of vitamin E, vitamin C, and garlic antagonized the inhibitory effect of CCl4 on GST. Vitamin E, vitamin C, and garlic decreased the oxidative stress measured as TBARs. Conclusion: vitamin C, vitamin E, and garlic exerted hepatoprotective effects against CCl4-induced toxicity.

PHD Title

EFFECT OF GREEN TEA AND/OR LICORICE ON LIVER OF ALBINO RATS INTOXICATED WITH DIMETHYLNITROSAMINE

PHD Abstract

Aim: This study was designed to investigate the effects of green tea and/or licorice aqueous extracts on antioxidant defense system, histopathological perturbations, and biochemical parameters related to liver and thyroid functions in DMN-intoxicated male albino rats. Methods: The tested extracts were administered ad-libitum to the rats for 4 weeks while DMN was injected (i.p.) seven times in the first 2 weeks of the start of the experiment. Results: It was found that DMN increased most of the antioxidant enzymes activities, while the tested extracts inhibited them. So, it can be hypothesized that the toxic metabolites produced by DMN metabolism induced those enzymes in order to scavenge the toxic metabolites and free radicals. Moreover, the withdrawal period may produce some degree of spontaneous recovery induced by compensatory mechanisms. In DMN-injected rats, it can be also hypothesized that the hepatoprotective effect of the tested extracts may be due to the alteration of drug-metabolizing enzymes more than due to activation of antioxidant enzymes without excluding the antioxidative capacity of the tested extracts themselves. DMN caused hepatobiliary inflammation and necrotic lesions as it estimated by histopathological studies and a profound rise of plasma CA 19.9 and bilirubin. DMN increased hepatic collagen and elastin contents indicating that DMN may cause liver fibrosis after further administration. On the other hand, the tested extracts decreased the hepatic collagen and elastin contents. So, they are considered to be cytoprotective agents against liver fibrosis. DMN showed degeneration, desquamation and atrophy of many acini of thyroid gland which was antagonized by the tested extracts as confirmed by the histological study. Conclusion: So, we can conclude that the tested extracts ameliorated the toxic effects resulted of DMN administration on liver and thyroid glands of male albino rats. Hence, the tested extracts can be used as a co-remedy of DMN toxicity. Key words Dimethylnitrosamine (DMN), green tea, licorice, antioxidants, lipid profiles, nitrite, CA 19.9, liver and thyroid function parameters, histopathological study and rats.